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Molecular Biology

PLANT GENOMIC DNA EXTRACTION - MINIPREP

CTAB RNA EXTRACTION (MAXI- & MINI-PREP)

SANGER SEQUENCING

RT-QPCR

TA CLONING (LIGATION, TRANFORMATION, COLONY PCR, MINIPREP)

PREPARATION OF E. COLI COMPETENT CELLS, ONE-STEP TSS METHOD

PREPARATION OF AGROBACTERIUM COMPETENT CELLS

PLASMID MINIPREP

Genome Editing

VARIANT-FREE, PRE-DESIGNED GRNAS FOR P. TREMULA X ALBA 717

GIBSON ASSEMBLY OF CRISPR VECTORS

AMPLICON SEQUENCING

Plant Transformation, Propagation, and Handling

TISSUE CULTURE MEDIA PREPARATION

717 TRANSFORMATION

ARABIDOPSIS SEED STERILIZATION AND PLATING

SOIL TRANSPLANTING

CUTTINGS

TISSUE GRINDING IN LIQUID NITROGEN

717 MICROPROPAGATION

Protein, Metabolite and other analysis

CRUDE PROTEIN EXTRACTION

NUCLEAR PROTEIN EXTRACTION

TUBULIN PROTEIN PURIFICATION

STARCH ANALYSIS

CONDENSED TANNIN ANALYSIS

SOXHLET EXTRACTION

GUS STAINING

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